ANNEX

QUALITY OF SHELLFISH WATERS // // // PARAMETER // G // I // REFERENCE METHODS OF ANALYSIS // MINIMUM SAMPLING AND MEASURING FREQUENCY // // // // // // // // // // // // // // // // // // // // // // // // // // // // // // // **** 1 . 2 . 3 . 4 . 5 . 6 . 7 . 8 . 9 . 10 . 11 . 12 . PH PH UNIT TEMPERATURE * C COLORATION ( AFTER FILTRATION ) MG PT/L SUSPENDED SOLIDS MG/L SALINITY PRO MILLE DISSOLVED OXYGEN SATURATION % PETROLEUM HYDROCARBONS ORGANOHALOGENATED SUBSTANCES // METALS // SILVER // AG // ARSENIC // AS // CADMIUM // CD // CHROMIUM // CR // COPPER // CU // MERCURY // HG // NICKEL // NI // LEAD // PB // ZINC // ZN // MG/L // FAECAL COLIFORMS/100 ML SUBSTANCES AFFECTING THE TASTE OF THE SHELLFISH SAXITOXIN ( PRODUCED BY DINOFLAGELLATES ) A DISCHARGE AFFECTING SHELLFISH WATERS MUST NOT CAUSE THE TEMPERATURE OF THE WATERS TO EXCEED BY MORE THAN 2 * C THE TEMPERATURE OF WATERS NOT SO AFFECTED 12 TO 38 PRO MILLE * 80 % THE CONCENTRATION OF EACH SUBSTANCE IN SHELLFISH FLESH MUST BE SO LIMITED THAT IT CONTRIBUTES , IN ACCORDANCE WITH ARTICLE 1 , TO THE HIGH QUALITY OF SHELLFISH PRODUCTS THE CONCENTRATION OF EACH SUBSTANCE IN SHELLFISH FLESH MUST BE SO LIMITED THAT IT CONTRIBUTES IN ACCORDANCE WITH ARTICLE 1 , TO THE HIGH QUALITY OF SHELLFISH PRODUCTS * 300 IN THE SHELLFISH FLESH AND INTERVALVULAR LIQUID ( 1 ) 7 - 9 A DISCHARGE AFFECTING SHELLFISH WATERS MUST NOT CAUSE THE COLOUR OF THE WATERS AFTER FILTRATION TO DEVIATE BY MORE THAN 10 MG PT/L FROM THE COLOUR OF WATERS NOT SO AFFECTED A DISCHARGE AFFECTING SHELLFISH WATERS MUST NOT CAUSE THE SUSPENDED SOLID CONTENT OF THE WATERS TO EXCEED BY MORE THAN 30 % THE CONTENT OF WATERS NOT SO AFFECTED THE CONCENTRATION OF EACH SUBSTANCE IN THE SHELLFISH WATER OR IN THE SHELLFISH FLESH MUST NOT EXCEED A LEVEL WHICH GIVES RISE TO HARMFUL EFFECTS ON THE SHELLFISH AND THEIR LARVAE THE SYNERGIC EFFECTS OF THESE METALS MUST BE TAKEN INTO CONSIDERATION CONCENTRATION LOWER THAN THAT LIABLE TO IMPAIR THE TASTE OF THE SHELLFISH GAS CHROMATOGRAPHY AFTER EXTRACTION WITH SUITABLE SOLVENTS AND PURIFICATION SPECTROMETRY OF ATOMIC ABSORPTION PRECEDED , WHERE APPROPRIATE , BY CONCENTRATION AND/OR EXTRACTION METHOD OF DILUTION WITH FERMENTATION IN LIQUID SUBSTRATES IN AT LEAST THREE TUBES IN THREE DILUTIONS . SUBCULTURING OF THE POSITIVE TUBES ON A CONFIRMATION MEDIUM . COUNT ACCORDING TO MPN ( MOST PROBABLE NUMBER ). INCUBATION TEMPERATURE 44 * C MORE OR LESS 0.5 * C EXAMINATION OF THE SHELLFISH BY TASTING WHERE THE PRESENCE OF ONE OF THESE SUBSTANCES IS PRESUMED QUARTERLY QUARTERLY QUARTERLY QUARTERLY MONTHLY MONTHLY , WITH A MINIMUM OF ONE SAMPLE REPRESENTATIVE OF LOW OXYGEN CONDITIONS ON THE DAY OF SAMPLING . HOWEVER , WHERE MAJOR DAILY VARIATIONS ARE SUSPECTED , A MINIMUM OF TWO SAMPLES IN ONE DAY SHALL BE TAKEN QUARTERLY HALF-YEARLY HALF-YEARLY QUARTERLY ABBREVIATIONS : G À GUIDE I À MANDATORY ( 1 ) HOWEVER , PENDING THE ADOPTION OF A DIRECTIVE ON THE PROTECTION OF CONSUMERS OF SHELLFISH PRODUCTS , IT IS ESSENTIAL THAT THIS VALUE BE OBSERVED IN WATERS IN WHICH LIVE SHELLFISH DIRECTLY EDIBLE BY MAN . * 40 PRO MILLE DISCHARGE AFFECTING SHELLFISH WATERS MUST NOT CAUSE THEIR SALINITY TO EXCEED BY MORE THAN 10 % THE SALINITY OF WATERS NOT SO AFFECTED * 70 % ( AVERAGE VALUE ) SHOULD AN INDIVIDUAL MEASUREMENT INDICATE A VALUE LOWER THAN 70 % , MEASUREMENTS SHALL BE REPEATED AN INDIVIDUAL MEASUREMENT MAY NOT INDICATE A VALUE OF LESS THAN 60 % UNLESS THERE ARE NO HARMFUL CONSEQUENCES FOR THE DEVELOPMENT OF SHELLFISH COLONIES HYDROCARBONS MUST NOT BE PRESENT IN THE SHELLFISH WATER IN SUCH QUANTITIES AS TO : PRODUCE A VISIBLE FILM ON THE SURFACE OF THE WATER AND/OR A DEPOSIT ON THE SHELLFISH , HAVE HARMFUL EFFECTS ON THE SHELLFISH THE CONCENTRATION OF EACH SUBSTANCE IN THE SHELLFISH WATER OR IN SHELLFISH FLESH MUST NOT REACH OR EXCEED A LEVEL WHICH HAS HARMFUL EFFECTS ON THE SHELLFISH AND LARVAE ELECTROMETRY MEASURED IN SITU AT THE TIME OF SAMPLING THERMOMETRY MEASURED IN SITU AT THE TIME OF SAMPLING FILTER THROUGH A 0.45 TEM MEMBRANE PHOTOMETRIC METHOD , USING THE PLATINUM/COBALT SCALE FILTRATION THROUGH A 0.45 TRM MEMBRANE , DRYING AT 105 * C AND WEIGHING CENTRIFUGING ( FOR AT LEAST FIVE MINUTES , WITH MEAN ACCELERATION 2 800 TO 3 200 G ), DRYING AT 105 * C AND WEIGHING CONDUCTIMETRY WINKLER ' S METHOD ELECTROCHEMICAL METHOD VISUAL EXAMINATION

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Source: EUR-Lex (Cellar) · retrieved 2026-10-13 · Text as adopted (Official Journal); later amendments are not incorporated in this text.