preamble_217
Council Directive 80/217/EEC of 22 January 1980 introducing Community measures for the control of classical swine fever · European Union
2. Virus isolation and identification in cell cultures (a) Virus isolation from tissue samples is performed on susceptible (PK 15) cell cultures or equally susceptible cell lines. Cell cultures grown on cover-slips are exposed to a suitably prepared 10 % suspension of tissue from the suspect animal ; starting with a 10 % suspension, the cultures shall be stained and examined for specific cytoplasmic fluorescence at intervals from 24 to 72 hours from the time of inoculation.
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Source: EUR-Lex (Cellar) · retrieved 2026-11-09 · Text as adopted (Official Journal); later amendments are not incorporated in this text.