preamble_234

Council Directive 80/217/EEC of 22 January 1980 introducing Community measures for the control of classical swine fever · European Union

The sera are diluted 1/5 for screening purposes. Two-fold dilutions of serum starting at 1/5 are prepared when a full titration is necessary. Each dilution is mixed with an equal volume of virus suspension containing 100 to 200 infectious doses (TCID 50). At least two cultures are used at each dilution level. The NIFT results are expressed as the reciprocal of the dilution at which half the inoculated cell cultures fail to show any specific fluorescence. An end-point between two dilution levels is interpolated.

← preamble_233 · All articles · preamble_237 →

Source: EUR-Lex (Cellar) · retrieved 2026-11-09 · Text as adopted (Official Journal); later amendments are not incorporated in this text.