preamble_2303

Council Directive 96/49/EC of 23 July 1996 on the approximation of the laws of the Member States with regard to the transport of dangerous goods by rail · European Union

The object is to determine the concentration which causes 50 % mortality in the test species; this is the (LC50) value, namely, the concentration of the substance in water which will cause the death of 50 % of a test group of fish during a continuous period of testing of at least 96 hours. Appropriate types of fish include: striped brill (Brachydanio rerio), fathead minnow (Pimephales promelas) and rainbow trout (Oncorhynchus mykiss).

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Source: EUR-Lex (Cellar) · retrieved 2026-10-31 · Text as adopted (Official Journal); later amendments are not incorporated in this text.